Vitamin B12 Crystalloid Powder, food grade

Catalog NumberDS68199-2

CAS Number68-19-9

CategoryVitamin B

Synonyms	Cyanocobalamin; Cyanocob(III)Alamin; Cobalamin; Cyanocobalamine
IUPAC Name	Cobalt(3+);[(2R,3S,4R,5S)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2R)-1-[3-[(1R,2R,3R,5Z,7S,10Z,12S,13S,15Z,17S,18S,19R)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7,12,17-tetrahydro-1H-corrin-24-id-3-yl]propanoylamino]propan-2-yl] phosphate;cyanide
Molecular Weight	1355.4
Molecular Formula	C63H88CoN14O14P
InChI	InChI=1S/C62H90N13O14P.CN.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;1-2;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);;/q;-1;+3/p-2/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57+,59-,60+,61+,62+;;/m1../s1
InChI Key	FDJOLVPMNUYSCM-WZHZPDAFSA-L
Melting Point	>300 °C
Purity	99%+
Appearance	Dark red crytalloid powder
Shelf Life	24 months
Storage	Store in a cool and dry place, away from light.
Certification	KOSHER/GMP/MUL/HALAL/
EC Number	200-680-0
Isomeric SMILES	CC1=CC2=C(C=C1C)N(C=N2)[C@@H]3[C@@H]([C@@H]([C@H](O3)CO)OP(=O)([O-])O[C@H](C)CNC(=O)CC[C@@]4([C@H]([C@@H]5[C@]6([C@@]([C@@H](/C(=C(/C7=N/C(=C\C8=N/C(=C(\C4=N5)/C)/[C@H](C8(C)C)CCC(=O)N)/[C@H]([C@]7(C)CC(=O)N)CCC(=O)N)\C)/[N-]6)CCC(=O)N)(C)CC(=O)N)C)CC(=O)N)C)O.[C-]
Packaging	20 kg
Standard	FCC, USP, EP
Type	Vitamin B12

Case Study

Vitamin B12 (Cyanocobalamin) Powder as a Labile Micronutrient Stabilized by Spray-Chilled Microencapsulation

Mazzocato, Marcella Chalella, et al. Food Research International 126 (2019): 108663.

Vitamin B12 (cyanocobalamin) powder is an essential water-soluble micronutrient required for red blood cell formation and nervous system function. However, free vitamin B12 powder is highly sensitive to light, heat, oxidizing agents, and neutral/alkaline pH, leading to significant degradation during storage and processing.
Experimental Protocol: Free vitamin B12 powder (0.1% and 1% purity) and solid lipid microparticles (SLM) loaded with vitamin B12 (0.1% or 1% vitamin, 0-5% soy lecithin, vegetable fat carrier) were prepared by spray chilling (atomisation at low temperature, 8.2 bar, cooling air at 5 °C). Particles were characterised for size (laser diffraction), morphology (SEM), encapsulation efficiency (HPLC), water activity, colour, X-ray diffraction, and in vitro release in simulated gastric fluid (SGF, pH 1.8) and simulated intestinal fluid (SIF, pH 6.5). Stability was monitored at 25 °C for 120 days.
Performance Evaluation: Free vitamin B12 powder retained only 75.2% (0.1%) and 76.6% (1%) after 120 days. In contrast, encapsulated vitamin retained >91% (up to 95.3% for formulation with 1% vitamin + 2.5% lecithin) under the same conditions. SLM were spherical, smooth, with mean diameter 13-27 μm, encapsulation efficiency 76-101%, and water activity 0.48-0.68 (microbiologically safe). X-ray diffraction confirmed stable β'-crystalline form with no polymorphic change. In SGF, lecithin-containing particles (5% lecithin) released only 8% vitamin in the first 10 min, achieving sustained release (~90% after 90 min), whereas particles without lecithin released 55-75% immediately. All formulations released rapidly in SIF. Spray-chilled encapsulation effectively protects vitamin B12 powder from degradation and enables controlled release.

Vitamin B12 (Cyanocobalamin) as a High-Dose Active Ingredient in Mucoadhesive Sublingual Tablets

Ma'ali, Anwar, et al. Pharmaceuticals 16.10 (2023): 1412.

Cyanocobalamin (vitamin B12) is a water-soluble, BCS Class III compound (high solubility, low permeability) with low oral bioavailability (1-5%). To enhance absorption, it was formulated into mucoadhesive sublingual tablets that increase residence time at the sublingual mucosa, bypassing first-pass metabolism.
Experimental Protocol: Tablets (50 mg, 1 mg cyanocobalamin) were prepared by direct compression using various polymers: HPMC (5%), HPC (15%), Eudragit L100-55 (15%), and xanthan gum (0.5%). Formulations were evaluated for pre-compression flow properties, tablet hardness, friability, disintegration time (<1 min), content uniformity, surface pH, ex vivo mucoadhesive residence time (bovine sublingual mucosa), mucoadhesive strength, drug release (dialysis membrane, simulated salivary fluid, pH 6.8, 22 h), and permeation (Franz diffusion cell, PBS pH 7.4, 6 h).
Performance Evaluation: Among 20 formulations, S11 showed the best performance: residence time 118.2 min, detachment force 26 g, drug content 99.1%, and surface pH 5.35 (non-irritating). Drug release reached 76.9% over 22 h, fitting the Peppas-Sahlin model (R² = 0.9949), indicating combined Fickian and non-Fickian release. Permeation across Permeapad gave a flux of 6.39 μg/h/cm² and apparent permeability (P_app) of 3.55×10^-6 cm/s, roughly twice that of HPMC tablets. The formulation significantly improved predicted bioavailability (~12%) compared to oral tablets (1.3%). Vitamin B12 powder thus benefits from sublingual mucoadhesive delivery using anionic polymers.

Custom Q&A

What is Vitamin B12 Crystalloid Powder and how is it used in food applications?

Vitamin B12 Crystalloid Powder, in its food-grade form, is a purified crystalline form of Vitamin B12 used to fortify foods and beverages. It ensures that products meet nutritional standards essential for human health, particularly in vegetarian and vegan products where natural sources of B12 might be absent. The crystalloid form is soluble, making it ideal for various food and beverage formulations.

Why is Vitamin B12 important in human nutrition?

Vitamin B12 is crucial for maintaining healthy nerve cells, supporting brain function, and aiding in the production of DNA and RNA. It plays a significant role in the formation of red blood cells and helps prevent megaloblastic anemia. Adequate intake is vital for energy metabolism and overall vitality.

What are the potential health implications of Vitamin B12 deficiency?

A deficiency in Vitamin B12 can lead to serious health issues, including neurological impairments, such as memory loss and difficulty walking, and hematological problems, like anemia. Other symptoms can include fatigue, weakness, and, eventually, neuropsychiatric disorders, making supplementation or fortified foods important for those at risk, such as the elderly or those with dietary restrictions.

How does the food-grade quality of Vitamin B12 Crystalloid Powder affect its application in food products?

The food-grade quality ensures that Vitamin B12 Crystalloid Powder is safe for consumption and meets regulatory guidelines for purity and efficacy. This quality allows it to be used widely across different food products without adversely affecting taste, texture, or safety, ensuring that consumers receive the intended health benefits.

Who would most benefit from foods fortified with Vitamin B12 Crystalloid Powder?

Individuals following vegetarian or vegan diets, the elderly, pregnant or breastfeeding women, and those with certain medical conditions such as pernicious anemia would see significant benefits from fortified foods. These groups are at a higher risk for Vitamin B12 deficiency due to limited dietary sources or increased nutritional needs.

What are the regulatory considerations for using Vitamin B12 in food products?

When fortifying foods with Vitamin B12, regulations from health authorities like the FDA or EFSA must be followed, ensuring that the levels used are both safe and effective. Labeling requirements must be adhered to, giving consumers important information about the source and quantity of the nutrient added.

In what types of food products is Vitamin B12 Crystalloid Powder commonly used?

It is commonly added to breakfast cereals, nutritional bars, plant-based milk alternatives, and dietary supplements. Its inclusion helps ensure these products support adequate vitamin intake, particularly for individuals who might not consume sufficient amounts through traditional dietary sources.

Our products and services are for research use only and cannot be used for any clinical purposes.

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